Appearance: white crystals or powder
1,10-Phenanthroline monohydrate 5144-89-8 is a metal chelating agent that prevents the induction of chromosomal aberrations in streptozotocin-treated cells. Acts as a general MMP (matrix metalloproteinase) inhibitor. Forms a red chelate with ferrous (iron II) ion that absorbs maximally at 510 nm. o-Phenanthroline has been employed as a photometric reagent for iron. o-Phenanthroline forms a complex with Fe2+, but not with Fe3+. NH2OH can be added to the solution to reduce the Fe3+ to Fe2+ for analysis of iron. Due to the o-Phenanthroline's strong UV absorbance, it may interfere with spectrophotometric assays. When complexed with copper, it possesses nuclease activity that has been employed in studying DNA-protein interactions.
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